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To test the mechanical integrity of silk broin in physiologic cell culture conditions, bundles of 30 extracted silk bers 3 cm between sutures were prepared Bundles were seeded as described above (See Protocol 84), using a 1 106 cells/ml cell suspension and cultured for 1, 7, 14, and 21 days (n = 8 per time point); otherwise identical nonseeded matrices were generated for use as controls Single pull-to-failure mechanical analysis was performed as described above by clamping directly on the knotted sutures, eliminating the need for molded epoxy ends; also, matrices were saturated with cell culture medium throughout the testing procedure The silk bers retained mechanical tensile strength over 21 days in tissue culture conditions, and no statistically signi cant changes in UTS were observed when comparing seeded and nonseeded matrices.

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versus deleted from the analysis (ie, they indicate by how many standard deviations each point changes each of the regression coef cients) We turn to our numerical example and examine the measures of leverage, discrepancy, and in uence for each case Table 198 summarizes the ndings To limit the demonstration, we adopt the strategy of identifying the case which is most extreme on each of the three diagnostic measures (In usual practice, we would adopt the strategy of identifying a few extreme cases on each diagnostic measure and examining them in detail or following some rules of thumb for selecting potential problematic cases) We also simplify matters by focusing on the limited regression equation of intention on centered risk, bene ts, and their interaction, summarized in Table 196, and given as Y = 3569 + 170 RISKC + 334 BENEFITC + .

19.8 27.7 32.1 45.2

Human BMSCs readily adhered, spread, and grew on the silk ber matrix after 1 day in culture and formed cellular extensions to bridge neighboring bers A.

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175 RISKC*BENEFITC The diagnostic measures identify two extreme cases, Cases 239 and 240 that together exhibit the most extreme scores on the three diagnostic measures Information about these cases is given in Table 198, Panel A As shown in Panel A(1), Case 240 is most extreme on the measures of leverage (hii) and overall in uence (DFFITS); Case 239 is most extreme on discrepancy (studentized deleted residual) Panel A(2) provides raw scores on risk, bene t, and intention Case 239 has the second-highest risk score in the sample (747 on a 10-point scale), a bene ts score at the 74th percentile of the sample, and a very low intention score (145 on a 6-point scale) at the 2nd percentile of intention in the sample Case 240 has the highest risk score in the sample (9.

experiencing an exercise high vary greatly Sachs (1997) estimated that somewhere between 9% and 78% of runners have experienced a runner s high Among runners who have experienced an exercise high, the frequency of such an experience varies from rarely (ie, only several times during their running careers) to an average of 294% of daily runs Because of its ability to enhance the quality of life, researchers need to further investigate the exerciser s high as well as provide information about factors that trigger this type of peak moment ENJOYMENT OF PHYSICAL ACTIVITY AND QUALITY OF LIFE Physical activity also contributes to quality of life by providing opportunities for enjoyment As emphasized by the burgeoning hedonic focus in psychology, enjoyable activities enhance our quality of life by providing interesting, rewarding, and truly memorable experiences (Kahneman, Diener, & Schwarz, 1999) As suggested by Csikszentmihalyi (1991, p.

46), When people ponder further about what makes their lives rewarding, they tend to move beyond pleasant memories and begin to remember other events, other experiences that overlap with pleasurable ones but fall into a category that deserves a separate name: enjoyment (italics added) Enjoyment: A Positive Affective State Enjoyment can be defined as an optimal psychological state (ie, flow) that leads to performing an activity for its own sake and is associated with positive feeling states (Kimiecik & Harris, 1996, p 256) Enjoyment often occurs in autotelic, or intrinsically rewarding activities The optimal experience of enjoyment seems to result in desirable affective states such as happiness, vigor, pleasure, and relaxation (Motl, Berger, & Leuschen, 2000) Enjoyment of physical activity also may produce feelings of accomplishment, euphoria, and happiness (Kendzierski & DeCarlo, 1991), which, in turn, may add meaning and zip to daily routines.

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