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the dif culty in obtaining a precise measurement of an analog voltage or current, either of which could be corrupted by noise or interference in a variety of ways, the probability of making an error in discerning between binary 0s and 1s is very small Further, as will be shown shortly, digital data is often coded in such a way that many transmission errors may be detected and corrected Finally, storage and processing of digital data are much more readily accomplished than would be the case with analog signals This section explores a few of the methods that are commonly employed in transmitting digital data; both parallel and serial interfaces are considered Digital signals in a microcomputer are carried by a bus, consisting of a set of parallel wires each carrying one bit of information In addition to the signalcarrying wires, there are also control lines that determine under what conditions transmission may occur A typical computer data bus consists of eight parallel wires and therefore enables the transmission of one byte; digital data is encoded in binary according to one of a few standard codes, such as the BCD code described in 13, or the ASCII code, introduced in 14 (see Table 146) This bus con guration is usually associated with parallel transmission, whereby all of the bits are transmitted simultaneously, along with some control bits Figure 1567 depicts the general appearance of a parallel connection Parallel data transmission can take place in one of two modes: synchronous or asynchronous In synchronous transmission, a timing clock pulse is transmitted along with the data over a control line The arrival of the clock pulse indicates that valid data has also arrived While parallel synchronous transmission can be very fast, it requires the added complexity of a synchronizing clock, and is typically employed only for internal computer data transmission Further, this type of communication can take place only over short distances (approximately 4 m) Asynchronous data transmission, on the other hand, does not take place at a xed clock rate, but requires a handshake protocol between sending and receiving ends The handshake protocol consists of the transmission of data ready and acknowledge signals over two separate control wires Whenever the sending device is ready to transmit data, it sends a pulse over the data ready line When this signal reaches the receiver, and if the receiver is ready to receive the data, an acknowledge pulse is sent back, indicating that the transmission may occur; at this point, the parallel data is transmitted Perhaps the most common parallel interface is based on the IEEE 488 standard, leading to the so-called IEEE 488 bus, also referred to as GPIB (for generalpurpose instrument bus) The IEEE 488 Bus The IEEE 488 bus, shown in Figure 1568, is an eight-bit parallel asynchronous interface that has found common application in digital instrumentation applications The physical bus consists of 16 lines, of which 8 are used to carry the data, 3 for the handshaking protocol, and the rest to control the data ow The bus permits connection of up to 15 instruments and data rates of up to 1 Mbyte/s There is a limitation, however, in the maximum total length of the bus cable, which is 20 m The signals transmitted are TTL-compatible and employ negative logic (see 13), whereby a logic 0 corresponds to a TTL high state (> 2 V) and a logic 1 to a TTL low state (< 08 V) Often, the eight-bit word transmitted over an IEEE 488 bus is coded in ASCII format (see Table 146), as illustrated in Example 1516.

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Data lines 0 1 2 3 4 5 6 7 Handshake lines DAV NRFD NDAC Control lines IFC ATN REN SRQ EOI Interface clear Attention Remote enable Service request End or identify Data valid Not ready for data Not data accepted

To determine whether a population is in Hardy-Weinberg proportions, the observed and expected distribution of genotypes can be compared by the chi-square statistical test. In some circumstances, when it is reasonable to assume equilibrium, we can estimate allelic and genotypic frequencies even when dominance occurs. The HardyWeinberg equilibrium is easily extended to the prediction of the frequencies of multiple alleles, multiple loci, and different frequencies of alleles in the two sexes, for both sexlinked and autosomal loci.

Known Quantities: Digital voltmeter reading, V Find: Binary data sequence Schematics, Diagrams, Circuits, and Given Data: V = 3405 V ASCII conversion

table (Table 146)

a 1/3

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where n is the number of ancestors in a given path and FJ is the inbreeding coef cient of the common ancestor of that path. Inbreeding exposes recessive deleterious traits already present in the population and causes homozygosity throughout the genome. It does not, by itself, change allelic frequencies. F can also be calculated from the reduction in heterozygosity in a population.

Analysis: Using Table 146, we can tabulate the conversion as follows:

The actual binary data sent can therefore be determined by converting the hex ASCII sequence into binary data (see 13): 33 2E 34 30 35 110011 101110 110100 110000 110101

Answer: Since the sample size is one hundred, the proportions of the three genotypes, SS, SF, and FF, are 0.66, 0.20, and 0.14, respectively. We can calculate allelic frequencies directly from these genotypes, remembering that the frequency of an allele is the frequency of its homozygote plus half the frequency of the heterozygote, or p q f(S) 0.66 f(F ) 0.14 f(SS) (1 2)f(SF ) (1 2)(0.20) 0.76 f(FF ) (1 2)f(SF ) (1 2)(0.20) 0.24

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melanogaster) from California were tested for their genotype at the alcohol dehydrogenase locus using starch-gel electrophoresis. Two alleles were present, S and F, for slow and fast migration, respectively. The following results were noted: SS, sixty-six; SF, twenty; FF, fourteen. What are the allelic and genotypic frequencies in this population

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