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13.3.1. Harvest of Murine ASCs from Inguinal Fat Pads Protocol 13.1. Harvest of Murine Adipose Tissue Reagents and Materials Sterile Hanks buffered salt solution (HBSS) ASC medium (see Section 13.2.1) Collagenase solution (see Section 13.2.3) Betadine iodine solution Petri dishes, 9 cm Centrifuge tube, 50 mL Tissue culture ask, 25 cm2 Scissors (2 pairs) Forceps (2 pairs) PBSA Nonsterile Mice, 4 6 Anesthetic: TBE (tribromoethylene) Water bath at 37 C Procedure (a) Warm HBSS and ASC medium in 37 C water bath. (b) Anesthetize and euthanize animals. (c) Transfer anesthetized animals to laminar ow hood. (d) Swab abdomen with 70% alcohol. (e) Open abdomen through low transverse incision. (f) Using forceps, remove gonadal/epididymal and inguinal fat pads. (g) Place fat in Petri dish with HBSS until all animals are sacri ced. (h) Transfer fat to new Petri dish. (i) Add HBSS + 5% Betadine iodine solution for 2 3 min. (j) Rinse tissue by aspirating Betadine solution and washing fat with HBSS. (k) Place fat in 50-mL centrifuge tube with sterile forceps. (l) Finely mince fat with sterile scissors. (m) Add one tissue volume of collagenase solution and mix. (n) Place tube in 37 water bath for 60 min, swirling occasionally. (o) Centrifuge sample at 50 100 g for 5 min. (p) Remove tube from centrifuge, shake vigorously (to complete separation of stromal cells from primary adipocytes), and centrifuge again for 5 min. (q) Carefully aspirate the oil on top, which includes primary adipocytes. Do not disturb the stromal-vascular fraction at the bottom.

(9.13)

Most modifications to tables start in the Property inspector. Dreamweaver helps you manage the basic table parameters width, border, and alignment and provides attributes for other useful but more arcane features of a table. These include converting table width from pixels to percentage of the screen, and vice versa.

The time has come to bring this chapter to a close with a look at just what frame relay can do to help resolve all of the issues regarding network limitations surveyed to this point. This section is not intended to replace the list of frame relay benefits given in the Introduction. Think of this more as the foundation for all the benefits listed earlier. There are four main benefits that frame relay networks offer to organizations. These are in terms of bandwidth, connectivity, economics, and applications. The concluding sections look at each one in turn.

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The configuration of options to match fields from the incoming data (or database) file to the field structure of a table in your solution requires that you select a destination table in your solution and then designate fields or values in the external file to align with some or all of the fields in the selected table, as shown in Figure 13.16. Individual fields in the Target Fields column at the right of the Import Field Mapping dialog can be moved up and down to correspond to incoming data by selecting them and using the + or Ctrl+ and + or Ctrl+ to move them up and down, or by dragging them with the handle icon to the left of each field. Moreover, you can click the arrow in the center column adjacent to any field to enable or disable import into a specific field.

Figure 11.7 illustrates the application. Note the various back-end sys tems that act as the sources for data and information to be displayed on the mobile device. In general, this simple yet very effective application allowed its users to make better decisions, faster. The wireless solution alerted our client s des ignated dashboard users on an exception basis. Whenever a certain metric, specified during the development of the solution by the various user groups involved in its design, was deviating from the standard, a systemgenerated message would be sent to the mobile device. Such deviations could be positive or negative in nature, exemplified by a large sales con tract that required immediate special pricing approvals, or the failure of production equipment that required immediate attention regarding its repair. In addition to system-generated alerts, the solution allowed for instant communication with dashboard users on the go. Although this functional ity is nothing special in our age of cellular communications, it acquires new meaning when such communication can be combined with the aforemen tioned actionable alerts. The operative word here is actionable. Being able to receive an alert, say, about an equipment malfunction, and then being able to work with that alert by responding to it, forwarding it, or

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The Quick Start screen has had several changes made to it in FileMaker 10. This screen appears when FileMaker Pro is first launched and when you choose File New Database command (provided the Show FileMaker Quick Start Screen option has not been disabled in the General tab of the FileMaker Preferences dialog). As shown in Figure 4.22, the Learn More group of links in the Quick Start screen is arranged into four categories in FileMaker Pro 10. This new arrangement provides ready access to new tutorial materials to provide an introduction to the main features of FileMaker Pro.

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