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2. 5
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where the integrals ha , Jab , and Kab were de ned in equations (A.19), (A.25), and (A.26), respectively. The orbital energy is the kinetic energy of a single electron with the distribution speci ed by the MO, its attraction to all of the nuclei, and its repulsion in an averaged way with all of the other electrons in the molecule. The total electronic energy in terms of the same integrals was de ned in equation (A.27) as EHF It is clear that EHF
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Magnusson, I, Rothman, DL, Katz, LD et al (1992) Increased rate of gluconeogenesis in type II diabetes mellitus A 13C nuclear magnetic resonance study The Journal of Clinical Investigation, 90 (4), 1323 7 Malik, S, Wong, ND, Franklin, SS et al (2004) Impact of the metabolic syndrome on mortality from coronary heart disease, cardiovascular disease, and all causes in United States adults Circulation, 110, 1245 50 Martin, ML and Jensen, MD (1991) Effects of body fat distribution on regional lipolysis in obesity The Journal of Clinical Investigation, 88, 609 13 Mathis, D, Vence, L and Benoist, C (2001) -cell death during progression to diabetes Nature, 414, 792 8 McCarthy, M and Zeggini, E (2006) Genetics of type 2 diabetes Current Report in Diabetes, 6, 147 54 McNeely, MJ, Boyko, EJ, Shofer, JB et al.
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are in van der Waals contact with other atoms and cannot be packed more densely due to the limitation imposed by their van der Waals contact distance The hydrophobic effect is another important stabilizing force in proteins This arises from folding of the protein to minimize exposure of hydrophobic residues to solvent water Strongly hydrophobic side-chains (eg phenylalanine) can only be dissolved in water at considerable energy cost because they would strongly disrupt the hydrogen-bonded structure of the solvent Moreover, the hydrophobic effect is largely entropic (rather than enthalpic) since removal of a hydrophobic residue from water releases solvent water from the constraints it had imposed leading to a net increase in solvent entropy Packing of the polypeptide chain to remove such residues from contact with water is therefore strongly thermodynamically favoured.
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